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Harvard Bioscience btx ecm 830 square wave electroporation system
Btx Ecm 830 Square Wave Electroporation System, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/btx+830/btx+ecm+830/pm40138313-426-12-20
Average 90 stars, based on 1 article reviews
btx ecm 830 square wave electroporation system - by Bioz Stars, 2026-09
90/100 stars

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Electroporation:

Article Title: Sevoflurane Inhibits the Proliferation of Neural Precursor Cells and Neural Migration of Mice by Inducing Iron Metabolism Disorders.
Article Snippet: Plasmid DNA and 0.01% fast green (Fluka) were injected into the embryonic brain's lateral ventricles by a glass micropipette. .. For electroporation, 5 × 50 ms, 37 V square pulses separated by 950 ms intervals were delivered with forceps- type electrodes connected to an ECM 830 electroporator (BTX Harvard Apparatus). ..

Article Title: Revisiting insulin-stimulated hydrogen peroxide dynamics reveals a cytosolic reductive shift in skeletal muscle.
Article Snippet: Furthermore, pLenti-FoxO1-Clover, a gift from Peter Rotwein (Addgene plasmid # 67759), was used to confirm insulin-stimulated Akt signaling in live imaging experiments [26]. .. FDB muscle electroporation was performed by delivering 15 pulses of 20 ms at 1 Hz at an electrical field strength of 75 V/cm using acupuncture needles (0.20 × 25 mm, Tai Chi, Lhasa OMS) connected to an ECM 830 BTX electroporator (BTX Harvard Apparatus). ..

Article Title: Sevoflurane Inhibits the Proliferation of Neural Precursor Cells and Neural Migration of Mice by Inducing Iron Metabolism Disorders
Article Snippet: Plasmid DNA and 0.01% fast green (Fluka) were injected into the embryonic brain's lateral ventricles by a glass micropipette. .. For electroporation, 5 × 50 ms, 37 V square pulses separated by 950 ms intervals were delivered with forceps‐type electrodes connected to an ECM 830 electroporator (BTX Harvard Apparatus). ..

Article Title: Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity
Article Snippet: .. 6 × 10 6 cells were then electroporated in a 2 mm path length electroporation cuvette (BTX Harvard Apparatus; Holliston, MA) with 5 μg of viral RNA using an ECM 830 Square Wave Electroporation System (BTX) at the following settings: five pulses, 99 μs per pulse, 1.1 s pulse intervals, 860 V. Following a ten-minute incubation at room temperature, the electroporated cells were seeded into 150 mm cell culture dishes and maintained in 5% FBS DMEM. ..

Injection:

Article Title: Top2b regulates morphological and migratory properties of retinal progenitor cells in vivo and upon transplantable matrix substrates
Article Snippet: The reporter pCAG-GFP plasmid solution with a concentration ranging 3–6 μg/μL and 0.2 μL of fast green (0.025%) (MilliporeSigma, Burlington, MA, USA, 2353-45-9) was attached to a 0.1 mL syringe (Hamilton Company, Reno, NV, USA, Gastight Model 1710) for visual confirmation of solution delivery and mounted onto a manual micromanipulator (WPI, Sarasota, FL, M3301-M3-R) with a small piece of masterflex silicone tubing on the syringe needle. .. The mixture was injected directly into the embryonic subretinal space, and the embryo was electroporated with 5 square pulses of 15 V for 50 ms with 950 ms intervals using a pulse generator (BTX Harvard Apparatus, Boston, MA, USA, ECM 830). ..

Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
Article Snippet: A pilot hole was made with a 30-G beveled needle, a 33-G blunt needle was positioned in the subretinal space, and ∼450 nL of plasmid DNA in PBS with 0.1% Fast Green dye was injected using a UMP3 Microsyringe Injector and Micro4 Controller (World Precision Instruments). .. Five square wave pulses (80 V, 50 ms with 950 ms-intervals) were applied with an ECM 830 square wave electroporator (BTX Harvard Apparatus) and custom tweezer-type electrodes, with the negative electrode on the injected eye. ..

Incubation:

Article Title: Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity
Article Snippet: .. 6 × 10 6 cells were then electroporated in a 2 mm path length electroporation cuvette (BTX Harvard Apparatus; Holliston, MA) with 5 μg of viral RNA using an ECM 830 Square Wave Electroporation System (BTX) at the following settings: five pulses, 99 μs per pulse, 1.1 s pulse intervals, 860 V. Following a ten-minute incubation at room temperature, the electroporated cells were seeded into 150 mm cell culture dishes and maintained in 5% FBS DMEM. ..

Cell Culture:

Article Title: Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity
Article Snippet: .. 6 × 10 6 cells were then electroporated in a 2 mm path length electroporation cuvette (BTX Harvard Apparatus; Holliston, MA) with 5 μg of viral RNA using an ECM 830 Square Wave Electroporation System (BTX) at the following settings: five pulses, 99 μs per pulse, 1.1 s pulse intervals, 860 V. Following a ten-minute incubation at room temperature, the electroporated cells were seeded into 150 mm cell culture dishes and maintained in 5% FBS DMEM. ..

other:

Article Title: Materials and methods for modifying
Article Snippet: The cuvette was placed in a BTX Harvard Apparatus Electro Square Porator (ECM 830) (Holliston, Mass.) and electroporated (voltage at 1.7 kV, pulse length at 176 us, pulse intervals at 100 ms, and number of pulses at 18 times).



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